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Bisphenol A (BPA) ELISA Test Kit

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  Post Date: Feb 19,2023
  Expiry Date: Feb 19,2024
  Detailed Description: Principle
Bisphenol A (BPA) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Bisphenol A in tissue,honey, milk, urine and Serum.
The coupling antigens are coated on the micro-well stripes. The Bisphenol A in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Bisphenol A antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the Bisphenol A in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Bisphenol A concentration in the sample.

Technical specification:
Sensitivity: 5 ppb Detect time: Approx.75 min

Detect limit
Urine,serum.................................... 10 ppb
Liver,tissue......................................7.5 ppb

Recovery rate
Urine,serum................................ 70 ± 10%
Liver,tissue..................................80 ± 10%

Cross-reaction rate
Bisphenol A .......................100%
Thiamphenicol........................< 0.1%
Florfenicol...............................< 0.1%

Components
1.Microplate: 12 strips with 8 removable wells each
2.6× standard solution (1 mL each):
0 ppb, 1ppb, 5 ppb, 15 ppb, 45 ppb, 135 ppb, 405 ppb,
3.Enzyme conjugate (1................................................... red cap
4.Antibody working solution (7 mL)......................................green cap
5.Substrate A solution (7 mL)......................................... white cap
6.Substrate B solution (7 mL).......................................... black cap
7.Stop solution (7 mL)................................................ yellow cap
8.Washing solution (40 mL, 20× ) ............................. transparent cap
9.Redissolving solution (50 mL, 2× )................................... blue cap


  Company: Wuhan Ecalbio Co.,Ltd     [ China ]        
  Contact: Ku
  Tel: 027-87745856
  Fax: -
  Email: info@ecalbio.com
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