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Quinolones(QNS) ELISA Kit

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  Post Date: Aug 02,2017
  Expiry Date: Aug 02,2018
  Detailed Description: Payment Method: T/T or Western Union
Quinolones(QNS) ELISA Kit
Catalog No. LSY-10016
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Quinolones in samples. The coupling antigens are pre-coated on the micro-well stripes. The Quinolones in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Quinolones antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Quinolones in the sample. This value is compared to the standard curve and the Quinolones concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 1 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit
Enrofloxacin (ENR) 1ppb
Ciprofloxacin (CIF) about 1ppb
Ofloxacin (OFL) about 1ppb
Danofloxacin (DAN) about 1ppb
Norfloxacin (NOR) about 0.5ppb
Lomefloxacin (LOM) about 1ppb
Pefloxacin (PEF) about 0.5ppb
Enoxacin (ENO) about 1ppb
Aosuo Li acid (OXO) about 1ppb
Fluoroquinolone acid (FLU) about 1ppb
Ma Paul Sand Star (MAR) about 1ppb
Ammonia difloxacin (AMI) about 1ppb
That difloxacin (NAD) about 2ppb
Cross-reaction rate:
Enrofloxacin (ENR) 100%
Ciprofloxacin (CIF) 92.88%
Ofloxacin (OFL) 98.86%
Aosuo Li acid (OXO) 116.86%
Danofloxacin (DAN) 102.63%
Norfloxacin (NOR) 148.65%
Lomefloxacin (LOM) 86.24%
Pefloxacin (PEF) 156.60%
Enoxacin (ENO) 98.97%
Fluoroquinolone acid (FLU) 96.73%
Ma Paul Sand Star (MAR) 110.63%
Ammonia difloxacin (AMI) 98.86%
That difloxacin (NAD) 56.81%
Recovery rate
Tissue 80±15%
Serum 80±15%
Honey 75±15%

3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0ppb, 1ppb, 3ppb, 9ppb, 27ppb and 81ppb
3) Enzyme conjugate (7ml) ………………………………………………….red cap
4) Antibody working solution (7 mL) blue cap
5) Substrate A solution (7 mL) white cap
6) Substrate B solution (7 mL) black cap
7) Stop solution (7 mL) yellow cap
8) 20× concentrated washing buffer (40 mL) white cap
9) 2× concentrated redissolving solution (50 mL) transparent cap


  Company: Shenzhen Lvshiyuan Biotechnology Co.,Ltd     [ China ]        
  Contact: Bella Zou
  Tel: +86-755-28438788
  Fax: +86-755-28938800
  Email: info@lsybt.com
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