Details for Calcein Blue
Calcein Blue
111Category: Organic chemicals and Derivatives
CAS NO: 54375-47-2 EC NO: 259-129-8 Molecular Formula: C15H14NO7 Molecular Weight: 320.2747 Specification: InChI: InChI=1/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)/p-1 Product description:
Storage: Ambient Temperature
Shipping Condition: Ambient Temperature
Chemical Name: 8-[N,N-Bis(carboxymethyl)aminomethyl]-4-methylumbelliferone
CAS Number: 54375-47-2
Appearance: white or pale yellow powder
Sensitivity: pass test
Sulfated ash: <0.5 %
Solubility: 1 g/25 ml, 1 M KOH100 ml water
Calcein Blue is a fluorescent metal indicator in EDTA titration. It is easily soluble in alkaline solutions, but barely soluble in water. Its proton dissociation constants are reported to be pKa1=2.45, pKa2=7.24 and pKa3=11.3. Calcein Blue solution emits bright blue
Appearance: white or pale yellow powder Sensitivity: pass test Sulfated ash: <0.5 % Solubility: 1 g/25 ml, 1 M KOHà100 ml water Calcein Blue is a fluorescent metal indicator in EDTA titration. It is easily soluble in alkaline solutions, but barely soluble in water. Its proton dissociation constants are reported to be pKa1=2.45, pKa2=7.24 and pKa3=11.3. Calcein Blue solution emits bright blue fluorescence (lem=445 nm) at pH 4-11, but no fluorescence at pH 12 or higher. The blue fluorescence of the Calcein Blue solution at pH 4-11 is quenched by metal ions such as Co, Cu, Mn, Ni and Pb. However, Calcein Blue forms a highly fluorescent complex with alkaline earth metals at pH 12 or higher. Since the endpoint of the chelate titration is clear by UV irradiation at 200-370 nm in a dark room, highly stained sample can be used. Calcein Blue is also suitable to determine Fe and Zr ions. Calcein Blue-Zn chelate can detect 10 ppb of F-. Calcein Blue aqueous solution emits laser at 449-490 nm.Molecular Structure: 
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