- Category :
Pharmaceuticals and Biochemicals/Biochemicals and biotech products
- CAS NO : 39450-01-6
- EC NO : 254-457-8
- Molecular Formula :
- Main Specifications : mg;g;bulk
- Synonyms : proteinase K from tritirachium album*molecular bi;proteinase K aseptically filled;proteinase K solution in 40%*(V/V) glycerol;proteinase K from tritrachium album;proteinase K crude;PROTEINASE K;PROTEINASE K, LYOPHILIZED;PROTEINASE K, IMMOBILIZED;;IUB: 3.4.21.64;IUB: 3.4.21.14;EC 3.4.21.14;EC 3.4.21.64;3.4.21.14;Recombinant Proteinase K;Proteinase K from Tritirachium album limber, Recombinant;
Package: Aluminum Foil Bag
Uses : A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl f
Molecular Structure:
Product description:
Recombinant Proteinase k
For research use only
9-Recombinant proteinase K.rar
ALTERNATE NAMES
Protease K, Endopeptidase K, Tritirachium alkaline proteinase
DESCRIPTION
A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
ADVANTAGES
High purity: ≥99%
Activity: >30 units/mg
DNAses and RNAses: not detected
MOL.WEIGHT
28.9 kDa
FORM
Lyophilized
UNIT DEFINITION
One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 mol tyrosine per min at 37°C, pH 7.5(color by Folin-Ciocalteu reagent).
RECONSTITUTION
In 50 mM Tris-HCl, 2 mM Calcium acetate, pH 8.0.
STORAGE CONDITIONS
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.
HANDLING
Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.