- Category :
Pharmaceuticals and Biochemicals/Biochemicals and biotech products
- CAS NO : 91932-65-9
- EC NO : 295-242-9
- Molecular Formula :
- Main Specifications : mg;g;bulk
- Synonyms : Staphylococcal Protein A;CCRIS 4112;Protein A;Recombinant Protein A;SPA;
Package: Aluminum Foil Bag
Uses : Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies
Product description:
Recombinant Protein A
No antibody contaminant. No antibody affinity column purification.
5-Recombinant protein A.rar
SOURCE
E.Coli, HPLC purified.
SEQUENCE
Protein A mutant of Staphylococcus aureus
MOL.WEIGHT
25 kDa
FORM
Lyophilized powder.
Solution:50mg/ml in deionized water or PBS.
PH STABLE RANGE
pH 1-13
STORAGE CONDITION
2℃~8℃.
APPLICATION
The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.
Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.
PRODUCT SPECIFICATION
Test/Method Specification
Bioburden No organisms detected.
12% SDS-PAGE Single major band
Coomassie Stain 25kD
UV Spectrum E270/250≥1.2,E0.1% at 270nm=0.14-0.20
UV spectrometry No DNA or RNA detected
Triton Content No Triton contaminant.
USAGE:
For Research or Manufacturing Purpose Only. Not for Humans.