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Uses : Enterokinase is a specific protease that cleaves lysineC-terminalpreceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. However, enterokinase does not work if this lysine is followed by a proline. Enterokinase can remove N-terminal fusion protein, it is ve

Product description: Recombinant Enterokinase High Specificity, High Purity 8-recombinant enterokinase.rar DESCRIPTION YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase is a specific protease that cleaves lysineC-terminalpreceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. However, enterokinase does not work if this lysine is followed by a proline. Enterokinase can remove N-terminal fusion protein, it is very useful for removing unwanted protein tags. ADVANTAGES High purity (Figure 1) （Figure 2） （Figure 3） Figure 1: One single main band by SDS-PAGE analysis Figure 2: SDS-PAGE analysis of 1 U EK effect on 50µg substrate after 0min, 10min, 20min, 30min, 40min, 50min and 60min, respectively Figure3: SDS-PAGE analysis of 1 U, 2U, 5U, 10U EK effect on 50µg substrate after 4h (Lane 1U, 2U, 5U and 10U), and 1 U EK effect on 50µg substrate after 16h (the right Lane 1U) respectively High specificity 1) Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK) 2) No any other contaminated proteases, no non-specific cutting sites. Conditions Cutting condition: given an example: 25mM Tris-HCl 8.0: Fusion protein concentration: 0.1-1mg/ml (total protein content: 50-100µg) EK content: 1-2U Temperature: 25℃ Time: overnight or 12h-16h for digestion. Common components influence the action of enterokinase ＞200mM imidazole or ＞200mM NaCl or ＞5% glycerin, the reaction may be effected. The following suggestions are given: 1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0. 2) If the dialysis is inconvenient, please dilute the sample to ＜100mM imidazole, ＜50mMNaCl, ＜5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:50µg fusion protein). 3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of EK in reaction system or extend the reaction time. MAIN FEATURES Source E.Coli M.W. Theoretical MW: 25,850 Da;The apparent MW on SDS-PAGE: about 27,000 Da Specific Activity One unit is defined as the amount of enzyme needed to cleave 50μg of fusion protein in 12 to16 hours to get 95% completion at 25°C in a buffer containing 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein. Storage Store at -20°C after delivery. Stability Keep cool with blue ice during shipping. Remained stable at 25°C for one week without activity lost. Can be stable after several times of freeze-thaw.