- Category :
Pharmaceuticals and Biochemicals/Biochemicals and biotech products
- CAS NO : 9025-24-5
- EC NO : 232-788-9
- Molecular Formula :
- Main Specifications : mg;g;bulk
- Synonyms : carboxypeptidase B from procine*pancreas protease;Carboxypeptidase B;Carboxypeptidase B, AEBSF treated hog pancreas;Recombinant Carboxypeptidase B,from E.coli;Sequencing recombinant Carboxypeptidase B,from E.coli;Carboxypeptidase B,recombinant from E.coli;Peptidy-L-lysine(L-arginine);protaminase;
Package: Aluminum Foil Bag
Uses : Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.
Product description:
Sequencing Grade Carboxypeptidase B
Animal Origin Free
SOURCE
Expressed in E. Coli, purified by HPLC.
DESCRIPTION
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
MAIN FEATURES
Source E.Coli
Purified by HPLC
Physical form White or white-like lyophilized powder
Specific activity NLT 200 units/mg pro.
No Contaminant activity No trypsin, chymotrypsin, carboxypeptidase A, or other proteases contaminant.
APPLICATION
Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.
Antibody quality control.
RECOMMEND USAGE
To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.5. The ratio to aimed protein is 1:500 to 1:1000 (w/w), the optimum pH is pH7-9.
STORAGE AND STABILITY
Recommend recombinant carboxypeptidase B lyophilized powder stored under 2-8℃ in sealed container. It is stable within 24 months.
After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.
RELATED PRODUCT: Recombinant Trypsin (sequencing purity), Recombinant Carboxypeptidase B (sequencing purity), V8 (sequencing purity), Recombinant Chymotrypsin (sequencing purity)