Uses : enzymic preparations

Product description: Malic dehydrogenase (MDH) exists in all organisms and is one of the key enzymes in biological sugar metabolism, catalyzing the reversible conversion between malic acid and oxaloacetate. MDH plays an important role in various physiological activities of cells, including energy metabolism in mitochondria and reactive oxygen species metabolism in plants, and has high theoretical research and economic significance. 1. In agricultural production, MDH is used to improve plant salt tolerance Aluminum toxicity in acidic soil is the main limiting factor for crop growth. Research has found that in order to improve the absorption of phosphorus elements in soil by plants, genetic engineering is used for plant molecular improvement breeding. For example, overexpression of MDH gene in alfalfa enhances the absorption of organic acids by transgenic alfalfa, thereby improving its adaptability to acidic soil and resistance to aluminum toxicity. However, when MDH is highly expressed, an increase in plant organic acid content can increase cell osmotic pressure and chelate to remove some ions, thereby improving plant salt tolerance. 2. Identifying the purity of tomato hybrids In tomato F1 hybrids, using electrophoresis to detect the differences in MDH isoenzyme zymogram to identify the purity of the hybrid has become a new time-saving and labor-saving detection method in seed production, which is conducive to identifying hybrid varieties with high yield, disease resistance, and stress resistance advantages, bringing significant economic benefits to agricultural production. 3. As a diagnostic antigen in medicine MDH has also attracted increasing attention in the field of medicine, such as the use of genetic engineering vaccines to prevent human taeniasis. Through bioinformatics analysis of the MDH gene of the Asian subspecies of Taenia saginata, it has been predicted that cytoplasmic MDH is a potential diagnostic antigen, providing important clues for the application prospects of Taenia in diagnosis, drug and vaccine research. Later, experiments proved that it can obtain highly efficient expression with immunological activity in the prokaryotic expression system, laying the foundation for further research on the function of this protein. At the same time, combining multiple recombinant vaccines to enhance vaccine protection has become the main direction for the development of edge worm vaccines in the future.