Shanghai Yaxin Biotechnology Co., Ltd.

Lipase A

• Category :

  Pharmaceuticals and Biochemicals/Biochemicals and biotech products

• CAS NO :

  9014-49-7

• EC NO :

• Molecular Formula :

• Molecular Weight :

• Main Specifications :

  mg;g;bulk

• Packing :

  Aluminum Foil Bag

• Product description :

  Recombinant Lipase A Biocatalyst with excellent enantioselectivity 6-Recombinant Lipase A.rar SOURCE E.Coli MOL.WEIGHT 65 kDa FORM Lyophilized powder, immobilized powder. DESCRIPTION Lipases (EC 3.1.1.3) are ubiquitous enzymes that catalyze the hydrolysis of fats and oils, and that play an important role in pharmaceutical. Lipases from Serratia marcescens (SML) are well-known for their excellent enantioselectivity in biocatalytic hydrolysis of trans-3-(4-methoxyphenyl) glycidic acid methyl ester [(±)-MPGM] to produce (2R, 3S)-3-(4-methoxyphenyl) glycidic acid methyl ester [(-)-MPGM], a key intermediate for the synthesis of diltiazem hydrochloride. APPLICATION 1) Hydrolysis of trans-3-(4-methoxyphenyl) glycidic acid methyl ester [(±)-MPGM] to produce (2R,3S)-3-(4-methoxyphenyl) glycidic acid methyl ester [(-)-MPGM]. 2) Hydrolysis of (±)-naproxen methyl ester to produce (-)-naproxen. 3) Hydrolysis of fatty acid esters. 4) Catalysis of ester substitution reaction. 5) Production of biodiesel. APPLICATION NOTES PH-stability pH 5~10 Thermal-stability Stable below 40°C, unstable at higher than 50°C. Stability against organic solvents Higher activity In 10%-50% DMSO, isopropyl ether, petroleum ether In 10%-25% ethanol, acetone and isopropanol In 90% isopropyl ether, petroleum ether 50% activity In 50% acetone and isopropanol Selectivity to different fatty-acid derivatives Priority to long-chain (> 10) fatty acid derivatives.

• Uses :

  1) Hydrolysis of trans-3-(4-methoxyphenyl) glycidic acid methyl ester [(±)-MPGM] to produce (2R,3S)-3-(4-methoxyphenyl) glycidic acid methyl ester [(-)-MPGM]. 2) Hydrolysis of (±)-naproxen methyl ester to produce (-)-naproxen. 3) Hydrolysis of fatty acid