111Category: | Intermediates |
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CAS NO: | 9028-12-0 | ||
EC NO: | 232-823-8 | ||
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Product description: Reaction: Acetophenone + NADPH = phenylethanol + NADP Application: Synthesis of enantiomerically pure alcohols with R-configuration Substrate specificity: Very broad substrate specificity: aliphatic, cyclic and aromatic ketones and diketones KM VALUE [mM]: 0.08 mM 2,4-pentadion, 0.34 mM 4-Cl-acetophenone at 30癈) and (Km 0.21 mM NADPH at 30癈 and pH 7.5 pH optimum: 6.5 Reduction and 9.0 oxidation Mr and structure: It is a homotetramer of four identical subunits with a molecular weight of 29.6 kDa Activity: 1500 U/ml at 30癈 with acetophenone as substrate Formulation: In glycerol Stability: Stable at20癈 Commentary: Mg2+ increase the activity Unit definition: 1 U corresponds to the amount of enzyme which reduces 1祄ol acetophenone per minute at pH 7.0 and 25癈 to phenylethanol |
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Synonyms: | EC 1.1.1.2;KETOREDUCTASE LB;KETOREDUCTASE T;KETOREDUCTASE CDX003;ALCOHOL DEHYDROGENASE CDX003;ALCOHOL DEHYDROGENASE LB;ALCOHOL DEHYDROGENASE, NADP+ DEPENDENT;ALCOHOL DEHYDROGENASE T; | ||
Molecular Structure: |