X-Zyme

R2-alcohol dehydrogenase

• Category :

  Intermediates

• Synonyms :

  EC 1.1.1.2;KETOREDUCTASE LB;KETOREDUCTASE T;KETOREDUCTASE CDX003;ALCOHOL DEHYDROGENASE CDX003;ALCOHOL DEHYDROGENASE LB;ALCOHOL DEHYDROGENASE, NADP+ DEPENDENT;ALCOHOL DEHYDROGENASE T;

• CAS NO :

  9028-12-0

• EC NO :

  232-823-8

• Molecular Formula :

• Molecular Weight :

• Main Specifications :

• Product description :

  Reaction: Acetophenone + NADPH = phenylethanol + NADP

  Application: Synthesis of enantiomerically pure alcohols with R-configuration

  Substrate specificity: Very broad substrate specificity: aliphatic, cyclic and aromatic ketones and diketones

  KM VALUE [mM]: 0.08 mM 2,4-pentadion, 0.34 mM 4-Cl-acetophenone at 30癈) and (Km 0.21 mM NADPH at 30癈 and pH 7.5

  pH optimum: 6.5 Reduction and 9.0 oxidation

  Mr and structure: It is a homotetramer of four identical subunits with a molecular weight of 29.6 kDa

  Activity: 1500 U/ml at 30癈 with acetophenone as substrate

  Formulation: In glycerol

  Stability: Stable at20癈

  Commentary: Mg2+ increase the activity

  Unit definition: 1 U corresponds to the amount of enzyme which reduces 1祄ol acetophenone per minute at pH 7.0 and 25癈 to phenylethanol

• Molecular Structure :