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Avian Marek's disease virus antibody ELISA kit

Inquiry
  Post Date: Aug 02,2017
  Expiry Date: Aug 02,2018
  Detailed Description: Specs:96 wells/kit
Price:200 USD Metric Tons
Payment Method: T/T or Western Union
Avian Marek's disease virus antibody ELISA kit
Catalog No. LSY-30016

1. Usage
This kit is used to detect Avian Marek's disease virus antibody in chicken serum, to assess antibody condition by Avian Marek's disease vaccine in chicken farm and assist diagnosis of serological infected chicken.

2. Principle
The Avian Marek's disease antibody ELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (450 nm).

3. Reagents
1) MD antigen coated microtiter strips 1 piece (96 wells)
2) Conjugate solution 1 bottle (12 mL/bottle)
3) 10× washing solution………………………………………1 bottle (50 mL/bottle)
4) Substrate A /B solution 1 tube each (6 mL/tube)
5) Sample dilution……………………………………………. 1 bottle (50 mL/bottle)
6) Stopping solution 1 tube (6 mL/tube)
7) Negative control 1 tube (800μL /tube)
8) Positive control 1 tube (800μL /tube)
9) Serum dilution plate……………………………………………………..…..1 piece
10) Adhesive film……………………………………………………………….2 pieces
11) Instruction……………………………………………………………………1 piece

4. Materials required but not provided
1) Micropipettors and disposable tips: 0.5μL~10μL、10μL~100μL、100μL~1000μL
2) 37 ℃ Incubator
3) Measuring cylinder: 500 ml
4) 96 wells microplate reader
5) Distilled water or deionied water
6) Bottle or microplate washing machine

5. Sample preparation
Take animal whole blood, make serum according to regular methods, the serum should be clear, have no hemolysis.


  Company: Shenzhen Lvshiyuan Biotechnology Co.,Ltd     [ China ]        
  Contact: Bella Zou
  Tel: +86-755-28438788
  Fax: +86-755-28938800
  Email: info@lsybt.com
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