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Bisphenol A (BPA) ELISA Test Kit
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Post Date: | Feb 19,2023 |
Expiry Date: | Feb 19,2024 |
Detailed Description: |
Principle
Bisphenol A (BPA) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Bisphenol A in tissue,honey, milk, urine and Serum. The coupling antigens are coated on the micro-well stripes. The Bisphenol A in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Bisphenol A antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Bisphenol A in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Bisphenol A concentration in the sample. Technical specification: Sensitivity: 5 ppb Detect time: Approx.75 min Detect limit Urine,serum.................................... 10 ppb Liver,tissue......................................7.5 ppb Recovery rate Urine,serum................................ 70 ± 10% Liver,tissue..................................80 ± 10% Cross-reaction rate Bisphenol A .......................100% Thiamphenicol........................< 0.1% Florfenicol...............................< 0.1% Components 1.Microplate: 12 strips with 8 removable wells each 2.6× standard solution (1 mL each): 0 ppb, 1ppb, 5 ppb, 15 ppb, 45 ppb, 135 ppb, 405 ppb, 3.Enzyme conjugate (1................................................... red cap 4.Antibody working solution (7 mL)......................................green cap 5.Substrate A solution (7 mL)......................................... white cap 6.Substrate B solution (7 mL).......................................... black cap 7.Stop solution (7 mL)................................................ yellow cap 8.Washing solution (40 mL, 20× ) ............................. transparent cap 9.Redissolving solution (50 mL, 2× )................................... blue cap |
Company: | Wuhan Ecalbio Co.,Ltd [ China ] |
Contact: | Ku |
Tel: | 027-87745856 |
Fax: | - |
Email: | info@ecalbio.com |
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