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Botulinum Toxin A Strip Test

Inquiry
  Post Date: Sep 10,2015
  Expiry Date: Sep 09,2016
  Detailed Description: Cas No. :RNS 92035 Specs:95%
Payment Method: tt
Product Description
REAGEN™ Botulinum Toxin A Strip Test is a qualitative and rapid lateral flow assay designed to detect Botulinum Toxin A residues in meat,feed,milk,honey,milk powder,soil,vomit,feces,serum and water sample. This test is designed for field use or reference lab settings. The unique features of the kit are:
 Rapid strip test method – 3 minutes
 High sensitivity (80ppb)
 High reproducibility

Procedure Overview
The assay uses a double antibodies sandwich colloidal gold based format. The sample is added to the test well to allow the Botulinum Toxin A binding protein on the NC membrane to engage with any Botulinum Toxin A present in the sample. The gold particles conjugated anti-Botulinum Toxin A will bind to the drug that are bind by the Botulinum Toxin A binding protein at the Test line (T-line), forming a signal (red line) at that position. The test is negative if no colored line appears in the T area.

Kit Contents, Storage and Shelf Life
The REAGEN™ Botulinum Toxin A Strip test has the capacity for 48 deter-minations. The shelf life is 24 months when the kit is properly stored.
Kit Contents Amount Storage
Reaction strip 48 strips 4°C-25°C
Manual 1 RT




Sample preparation

Meat/Feed/Sauce/Milk powder
1. Grind and mix a representative sample(according to accepted sample techniques)
2. Weight out 2 g of the ground sample and place into a suitable container.
3. Add 5ml stroke-physiological saline solution and shake for 10 minutes with a vortex.
4. Centrifuge sample for 5 minutes at 4000 rpm.
5. Use the 200μL supernatant per well for the test.

Honey/Milk/Serum
1. Take 0.1ml sample into a 2ml tube , add 0.4ml stroke-physiological saline solution and shake for 1 minutes with a vortex.
2. Use the 200μL supernatant per well for the test.

Soil/Vomit/Feces
1. Weight out 1 g of the ground sample and place into a suitable container.
2. Add 5ml stroke-physiological saline solution and shake for 10 minutes with a vortex.
3. Centrifuge sample for 5 minutes at 4000 rpm.
4. Use the 200μL supernatant per well for the test.

Water
1. Use the 200μL sample per well for the test.


  CAS Registry Number:

RNS 92035


  Company: REAGEN LLC     [ United States ]        
  Contact: Nicole
  Tel: 8568585434
  Fax: 1-302-269-1897
  Email: reagenkits@gmail.com
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