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Cephalexin ELISA Kit

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  Post Date: Feb 19,2023
  Expiry Date: Feb 19,2024
  Detailed Description: 1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Cephalexin in the tissue, liver, honey, intestine, shrimp, fish, milk, etc. The coupling antigens are pre-coated on the micro-well stripes. The Cephalexin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Cephalexin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Cephalexin concentration in the sample. This value is compared to the standard curve and the content of the corresponding Cephalexin is subsequently obtained.

2. Technical specifications
Sensitivity : 1 ppb Detect time: Approx. 75 min

Detection limit:
Tissue..............................4 ppb
Milk ..............................40 ppb

Recovery rate:
Tissue, milk.................85 ±10%

Cross-reaction rate:
Cephalexin ....................100%

3. Components
1）Microplate: 12 strips with 8 removable wells each
2）6× standard solution (1 mL each):0 ppb, 1ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb
3）Enzyme conjugate (7 mL)................................. red cap
4）Antibody solution (7 mL)................................. green cap
5）Substrate A solution (7 mL)..............................brown cap
6）Substrate B solution (7 mL).............................. black cap
7）Stop solution (7 mL)......................................... yellow cap
8）Washing solution (40 mL 20× )......................... white cap
9）Redissolving solution (50 mL 2×)..................... blue cap


  Company: Wuhan Ecalbio Co.,Ltd     [ China ]        
  Contact: Ku
  Tel: 027-87745856
  Fax: -
  Email: info@ecalbio.com
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