Dexamethasone ELISA Test Kit
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| Post Date: | Sep 10,2015 |
| Expiry Date: | Sep 09,2016 |
| Detailed Description: |
Cas No. :RND99059
Payment Method: tt Product Description REAGEN™ Dexamethasone ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Dexamethasone, Betamethasone, Flumethasone in meat, fish, shrimp,urine and serum. The unique features of the kit are: High recovery (80-105%), rapid (less than 30 minutes), and cost-effective extraction methods. High sensitivity (0.1 ng/g or ppb) and low detection limit (e.g. 0.1 ng/g or ppb for meat). High reproducibility. A quick ELISA assay (less than 2 hours regardless of number of samples). Procedure Overview REAGEN™ Dexamethasone ELISA Test Kit is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. Kit Contents, Storage and Shelf Life REAGEN™ Dexamethasone ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C . The shelf life is 12 months when the kit is properly stored. Kit Contents Amount Storage Dexamethasone-Coated Plate 1 x 96-well plate (8 wells x 12 strips) 2-8℃ Dexamethasone Standards Negative control (white CAP tube) 0.1 ng/mL (yellow CAP tube) 0.2 ng/mL (orange CAP tube) 0.4ng/mL (pink CAP tube) 0.8ng/mL (purple CAP tube) 1.6 ng/mL (blue CAP tube) 100 ng/mL(spiking, optional, red cap tube) 1mL 1mL 1mL 1mL 1mL 1mL 1mL 2-8℃ Dexamethasone Antibody #1 15mL 2-8℃ 100×HRP-Conjugated Antibody #2 2.5mL Antibody #2 Diluent 20mL 20× Wash Solution 30mL Stop Buffer 15mL TMB Substrate 15mL Sample Balance Buffer I 10 mL Sample Balance Buffer Ⅱ 62g 10×Sample Extraction Buffer 10 mL If you are not planning to use the kit for over 1 month ,Dexamethasone Antibody #1 and 100× HRP-Conjugated Antibody #2 at －20℃ or in a freezer is recommended. Sensitivity Sample Type Detection Limit (ng/g or ppb) Meat/Fish/Shrimp 0.1 Serum 0.5 Urine 0.5 Specificity Analytes Cross-Reactivity (%) Dexamethasone 100 Betamethasone 100 Flumethasone 100 Prednisolone 50 Prednisone 10 Methylprednisolone <1 Stanozolol <0.1 Required Materials Not Provided With the Kit Microtiter plate reader (450 nm) Constant temperature incubator Vortex mixer, (e.g. Gneie Vortex mixer from VWR) Tissue Mixer (e.g. Omni TissueMaster Homogenizer) Pressure Blowing Concentrator 10, 20, 100 and 1000 L pipettes Multi-channel pipette: 50-300 L (Optional) |
| CAS Registry Number: | RND99059 |
| Company: | REAGEN LLC [ United States ] |
| Contact: | Nicole |
| Tel: | 8568585434 |
| Fax: | 1-302-269-1897 |
| Email: | reagenkits@gmail.com |
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