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Erythromycin ELISA Test Kit
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| Post Date: | Feb 19,2023 |
| Expiry Date: | Feb 19,2024 |
| Detailed Description: |
1. Principle
Erythromycin ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Erythromycin in milk, honey and meat.The coupling antigens are coated on the micro-well stripes. The Erythromycin in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Erythromycin antibodies.After the addition of the enzyme conjugate, the TMB substrate is added for coloration.The optical density (OD) value of the sample has a negative correlation with the Erythromycin in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Erythromycin concentration in the sample. 2. Technical specification: Sensitivity: 0.1 ppb Detect time: Approx.75 min Detect limit: Chicken,duck, pork, shrimp,fish,honey and milk .................4 ppb Cross-reaction rate: Erythromycin.............................................................. 100% Erythromycin Thiocyanate ................................................ 105% Erythromycin Ethylsuccinate ............................................ 85% Recovery rate: Tissue ............................................................... 85 ± 20% Honey, milk .......................................................... 75 ± 20% 3. Components 1） Microplate: 12 strips with 8 removable wells each 2） 6× standard solution (1 mL each): 0ppb、0.1 ppb、0.3 pb、 0.9 ppb、2.7 ppb、8.1ppb 3） Enzyme conjugate (12 mL) ............................................ red cap 4） Antibody solution (7 mL) ........................................ green cap 5） Substrate A solution (7 mL) ..................................... white cap 6） Substrate B solution (7 mL) ....................................... black cap 7） Stop solution (7 mL)............................................. yellow cap 8） Washing solution (40 mL, 20× Concentrate).................transparent cap 9） Buffer 1 (50 mL, 2× Concentrate)..................................blue cap |
| Company: | Wuhan Ecalbio Co.,Ltd [ China ] |
| Contact: | Ku |
| Tel: | 027-87745856 |
| Fax: | - |
| Email: | info@ecalbio.com |
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