Fructose/Glucose Assay Kit
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| Post Date: | Sep 10,2015 |
| Expiry Date: | Sep 09,2016 |
| Detailed Description: |
Cas No. :RNF91002
Product Description
REAGEN™ Fructose/Glucose Assay Kit is a cuvette-based enzymatic assay for the determination of Fructose/Glucose across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Fructose/Glucose directly from food samples. The unique features of the kit are: High sensitivity and low detection limit (0.8 ppm) High reproducibility A rapid (30 minute), robust enzyme-based assay Procedure Overview REAGEN™ Fructose/Glucose Assay Kit uses a coupled enzymatic reaction to detect glucose and/or fructose in food samples: glucose is detected by phosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). This compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of glucose in the sample. Fructose is detected in similar manner after it is specifically converted to glucose-6-phosphate (G-6-P) using the enzyme phosphoglucose isomerase (PGI). The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of glucose in each sample is then directly determined from the change in absorbance at 340 nm within 30 minutes. |
| CAS Registry Number: | RNF91002 |
| Company: | REAGEN LLC [ United States ] |
| Contact: | nicole |
| Tel: | 8566711789 |
| Fax: | 8568585434 |
| Email: | reagenllc@gmail.com |
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