Fructose/Glucose Assay Kit
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| Post Date: | Sep 10,2015 |
| Expiry Date: | Sep 09,2016 |
| Detailed Description: |
Cas No. :RNF91002
Specs:95% Payment Method: tt Product Description REAGEN™ Fructose/Glucose Assay Kit is a cuvette-based enzymatic assay for the determination of Fructose/Glucose across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Fructose/Glucose directly from food samples. The unique features of the kit are: High sensitivity and low detection limit (0.8 ppm) High reproducibility A rapid (30 minute), robust enzyme-based assay Procedure Overview REAGEN™ Fructose/Glucose Assay Kit uses a coupled enzymatic reaction to detect glucose and/or fructose in food samples: glucose is detected by phosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). This compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of glucose in the sample. Fructose is detected in similar manner after it is specifically converted to glucose-6-phosphate (G-6-P) using the enzyme phosphoglucose isomerase (PGI). The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of glucose in each sample is then directly determined from the change in absorbance at 340 nm within 30 minutes. Kit Contents, Storage and Shelf Life REAGEN™ Fructose/Glucose Assay Kit has the capacity for 48 determinations or 24 determinations in duplicate. Store the kit at 4°C. The shelf life of the kit is 12 months, after receipt, when the kit is properly stored. Kit Contents Amount Storage Dilution Buffer 10 mL 4ºC Substrate Mix 20 mL 4ºC Hexokinase 0.7 mL 4ºC PGI Enzyme 0.2 mL 4ºC Microtiter Plate One 4ºC Sensitivity (Detection Limit) Sample Volume Detection Limit (mg/L) 0.01 mL 8 0.1mL 0.8 The linear range of the assay is 0.8 – 1000 mg/L (ppm). Required Materials Not Provided With the Kit Spectrophotometer (340 nm) Low volume (0.3 mL) UV cuvettes, such as Brand Scientific catalog #7592 00 Deionized or distilled water 50 mL plastic conical tubes Warnings and Precautions Do not use the kit past the expiration date. Try to maintain a laboratory temperature of 20°–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays in direct sunlight, as this may cause excessive heat and evaporation. Cold bench tops should be avoided by placing several layers of paper towel or some other insulate material under the assay plates during incubation. Make sure you are using only distilled or deionized water since water quality is very important. When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic. |
| CAS Registry Number: | RNF91002 |
| Company: | REAGEN LLC [ United States ] |
| Contact: | Nicole |
| Tel: | 8568585434 |
| Fax: | 1-302-269-1897 |
| Email: | reagenkits@gmail.com |
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