Glucose Assay Kit

　　Product DescriptionREAGEN™Glucose Assay Kit is a cuvette-based enzymatic assay for the determination of Glucose across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Glucose directly from food samples.The unique features of the kit are:

　　High sensitivity and low detection limit (0.8 ppm)High reproducibilityA rapid (30 minute), robust enzyme-based assay

　　Procedure OverviewREAGEN™Glucose Assay Kit uses a coupled enzymatic reaction to detect glucose and/or fructose in food samples: glucose is detected by phosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). This compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of glucose in the sample.

　　The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of glucose in each sample is then directly determined from the change in absorbance at 340 nm within 30 minutes.

RNF91001