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HPLC empty analytical column

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  Post Date: Jul 30,2013
  Expiry Date: Jul 30,2014
  Detailed Description: Payment Method: HPLC empty analytical column
Detailed Product Description


Fuji padding has stable performance, lower column pressure,column efficiency moderate, high cost performance .


HPLC columns are packed with very fine particles (usually a few microns in diameter). The very fine particles are required to attain the low dispersion that give the high plate counts expected of modern HPLC.



Plate counts in excess of 25,000 plates per column are possible with modern columns, however, these very high efficiencies are very rarely found with real samples because of the dispersion associated with injection valves, detectors, data acquisition systems and the dispersion due to the higher molecular weight of real samples as opposed to the common test samples.



Packing these small particles into the column is a difficult technical problem but even with good packing a great amount of care must be given to the column end fittings and the inlet and outlet connection to keep dispersion to a minimum. Some state of the art systems are now 'chip' based and may use no particles at all. Some limited use has been made of HPLC for preparative purposes using half inch to one inch diameter columns.



LC columns, in general, achieve their separation by exploiting the different intermolecular forces between the solute and the stationary pahse and those between the solute and the mobile phase. The column will retain those substances that interact more strongly with the stationary pahse than those that interact more strongly with the mobile phase. The basic intermolecular forces that are exploited in the HPLC are the same as those discussed in The Mechanism of Chromatographic Retention and The Thermodynamics of Chromatography of the Chrom-Ed series.



The main consideration with HPLC is the much wider variety of solvents and packing materials that can be utilized as because of the much lower quantities of both which are required. In particular very expensive optically pure compounds can be used to make Chiral HPLC stationary phases and may even be used as (disposable) HPLC solvents.

Chromatorex 10um C18 Liquid Column

Inner Diameter Column Length

100mm 125mm 150mm 200mm 250mm 300mm
φ4.6mm US$151 US$157 US$169 US$185 US$200 US$215
φ5.0mm US$169 US$185 US$192 US$200 US$208 US$223
φ10mm US$385 US$438 US$462 US$505 US$555 US$618
φ15mm US$585 US$654 US$715 US$846 US$985 US$1,115
φ20mm US$1,377 US$1,462 US$1,515 US$1,738 US$1,931 US$2,003
φ30mm US$2,538 US$2,646 US$2,800 US$3,108 US$3,262 US$3,415

The price will change as the exchange rate.


  Company: Dalian Replete Science And Technology CO.,LTD     [ China ]        
  Contact: Helen Sun
  Tel: 0411-83786041
  Fax: 0411-83786043
  Email: 83789041@163.com
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