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LSY-10051 Doxycycline ELISA Test Kit

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  Post Date: Aug 02,2017
  Expiry Date: Aug 02,2018
  Detailed Description: Specs:96 wells/kit
Price:250 USD Metric Tons
Payment Method: T/T or Western Union
Doxycycline ELISA Test Kit
Catalog No. LSY-10051
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Doxycycline in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Doxycycline in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Doxycycline antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Doxycycline in it. This value is compared to the standard curve and the Doxycycline concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.2 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Tissue, honey, liquid Milk(method 1) about 5ppb
Liquid Milk(method 2) about 10ppb
Serum about 2ppb
Note: ppb=ng/mL or ng/g
Cross-reaction rate:
Doxycycline 100%
Tetracycline 100%
Minocycline 100%
Chlortetracycline 180%
Oxytetracyline 40%
Recovery rate:
90±15%

3. Components
1)Micro-well strips: 12 strips with 8 removable wells each
2)10× concentrated standard solution: 0 ppb, 2ppb, 6ppb, 18ppb, 54ppb (0.5ml/bottle)
3)11X Concentrated Enzyme conjugate (0.7 mL)
4)Enzyme conjugate dilution (7 mL)
5)Substrate A (7 mL)
6)Substrate B (7 mL)
7)Stop solution (7 mL)
8)20× concentrated washing buffer (30 mL)
9)20× concentrated redissolving solution (10 mL)


  Company: Shenzhen Lvshiyuan Biotechnology Co.,Ltd     [ China ]        
  Contact: Bella Zou
  Tel: +86-755-28438788
  Fax: +86-755-28938800
  Email: info@lsybt.com
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