Melamine ELISA Test Kit
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| Post Date: | Aug 02,2017 |
| Expiry Date: | Aug 02,2018 |
| Detailed Description: |
Payment Method: T/T or Western Union Melamine ELISA Test Kit Catalog No. LSY-10026 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Melamine in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Melamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Melamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Melamine concentration in the sample. This value is compared to the standard curve and the Melamine concentration is subsequently obtained. 2. Technical specifications Sensitivity: 1 ppb Incubator temperature: 25℃ Incubator time: 30min～15min Detection limit: Milk powder 20 ppb Milk 16 ppb Milk/Milk powder (Method two) 1 ppb Tissue (chicken, pork, duck, fish, shrimp and liver) 2 ppb Feed 100 ppb Egg 20 ppb Serum 4 ppb Cross-reaction rate: Melamine 100% Cyanuric acid 60% Triazine <1% Diamino atrazine <1% Recovery rate: Milk/Milk powder 90±19% Tissue 85±20% Feed 85±20% Egg 80±20% 3. Components 1） Micro-well strips: 12 strips with 8 removable wells each 2） 6× standard solution (1 mL each): 0 ppb,1 ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb 3） Enzyme conjugate (7 mL) red cap 4） Antibody working solution (7 mL) blue cap 5） Substrate A solution (7 mL) white cap 6） Substrate B solution (7 mL) black cap 7） Stop solution (7 mL) yellow cap 8） 20× concentrated washing buffer (40 mL) white cap 9） 2× concentrated redissolving solution (50 mL) transparent cap |
| Company: | Shenzhen Lvshiyuan Biotechnology Co.,Ltd [ China ] |
| Contact: | Bella Zou |
| Tel: | +86-755-28438788 |
| Fax: | +86-755-28938800 |
| Email: | info@lsybt.com |
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