Nalidixic acid ELISA test kit
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| Post Date: | Sep 10,2015 |
| Expiry Date: | Sep 09,2016 |
| Detailed Description: |
Cas No. :RND99063
Price:300 USD Metric Tons Payment Method: tt Product Description REAGEN™ Nalidixic acid ELISA test kit enables government agencies, food manufacturers and quality assurance organizations to detect nalidixic acid to as low as 5ppb level in various sample types and to satisfy customer concerns about food safety. The features of the kit are: Rapid extraction method for shrimp/fish/meat samples with a high recovery rate of 75 - 105%. A quick ELISA assay (less than 50min regardless of number of samples). High reproducibility. Procedure Overview The method is based on a competitive colorimetric ELISA assay. The Nalidixic Acid Antibody has been coated in the plate wells. During analysis, sample is added along with the Nalidixic Acid-horseradish peroxidase (Nalidixic Acid-HRP) conjugate. If the nalidixic acid residue is present in the sample, it will compete for the Nalidixic Acid Antibody, thereby preventing the Nalidixic Acid-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the nalidixic acid residue concentration in the sample. Kit Contents, Storage and Shelf Life REAGEN™ Nalidixic acid ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored. Kit Contents Amount Storage Nalidixic acid anti-coated Plate 1 x 96-well plate (8 wells x 12 strips) 2-8°C Nalidixic acid Standards: Negative control (white cap tube) 0.5 ng/mL (yellow cap tube) 1.5 ng/mL (orange cap tube) 5 ng/mL (pink cap tube) 15 ng/mL (purple cap tube) 50 ng/mL (blue cap tube) 1000 ng/mL (spiking, optional, red cap tube) 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 2-8°C Nalidixic acid-HRP 12 mL 2-8°C 10X Sample Extraction Buffer ** 25 mL 20X Wash Solution ** 30 mL Stop Buffer ** 14 mL TMB Substrate ** 12 mL *If you are not planning to use the kit for over 3 months, store Nalidixic acid-HRP at -20°C preferably or in a freezer. Sensitivity (Detection Limit) Sample Type Detection Limit (ng/g or ppb) Fish/Shrimp/Meat 5 Specificity (Cross-Reactivity) Analytes Cross-Reactivity (%) Nalidixic acid 100 Danofloxacin 65 Enoxacin 40 Pipemidic acid 30 Ofloxacin 28 Benofloxacin 15 Oxolin acid 10 Required Materials Not Provided With the Kit Microtiter plate reader (450 nm) Incubator Tissue Mixer (e.g. Omni Tissue Master Homogenizer) Vortex mixer (e.g. Gneie Vortex mixer from VWR) 10, 20, 100 and 1000 L pipettes Multi-channel pipette: 50-300 L (Optional) |
| CAS Registry Number: | RND99063 |
| Company: | REAGEN LLC [ United States ] |
| Contact: | Nicole |
| Tel: | 8568585434 |
| Fax: | 1-302-269-1897 |
| Email: | reagenkits@gmail.com |
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