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Neomycin (NM) ELISA kit
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| Post Date: | Feb 19,2023 |
| Expiry Date: | Feb 19,2024 |
| Detailed Description: |
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Neomycin in the tissue, liver, honey, intestine, shrimp, fish, etc.The coupling antigens are pre-coated on the micro-well stripes. The Neomycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Neomycin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Neomycin concentration in the sample. This value is compared to the standard curve and the content of the corresponding Neomycin is subsequently obtained. 2. Technical specifications Sensitivity : 0.5 ppb Detect time: Approx. 75 min Detection limit: Tissue, liver, honey, shrimp, fish, milk ........... 10 ppb Recovery rate: Tissue, liver, honey, shrimp, fish, milk....... 85 ±10% Cross-reaction rate: Neomycin .......................100% Streptomycin................... < 1 % Gentamicin ..................... < 1 % 3. Components 1）Microplate: 12 strips with 8 removable wells each 2）6× standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb, 40.5 ppb 3）Enzyme conjugate (12 mL)................................red cap 4）Antibody solution (7 mL)................................ green cap 5）Substrate A solution (7 mL)............................. brown cap 6）Substrate B solution (7 mL)............................. black cap 7）Stop solution (7 mL)........................................ yellow cap 8）Washing solution (40 mL 20× )........................ white cap 9）Redissolving solution (50 mL 2×)..................... blue cap |
| Company: | Wuhan Ecalbio Co.,Ltd [ China ] |
| Contact: | Ku |
| Tel: | 027-87745856 |
| Fax: | - |
| Email: | info@ecalbio.com |
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