Nitrate/Nitrite Assay Kit
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| Post Date: | Sep 10,2015 |
| Expiry Date: | Sep 09,2016 |
| Detailed Description: |
Cas No. :RNF91004
Specs:95% Payment Method: tt Product Description REAGEN™ Nitrate/Nitrite Assay Kit is a cuvette-based enzymatic assay for the determination of Nitrate/Nitrite across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Nitrate/Nitrite directly from food samples. The unique features of the kit are: High sensitivity and low detection limit (0.02 ppm) High reproducibility A rapid (45 minute), robust enzyme-based assay Procedure Overview REAGEN™ Nitrate/Nitrite Assay Kit uses a coupled enzymatic reaction scheme to detect nitrate and/or nitrite in food samples: nitrate in the sample is converted by nitrate reductase enzyme to make nitrite. The nitrite then reacts with sulfanilamide and N-(1-naphthyl)-ethylene-diamine to form a red diazo dye. This dye, measured at 540 nm, is proportional to the level of nitrate and/or nitrite in the sample. The absorbance of each sample at 540 nm is measured using a spectrophotometer. The concentration of nitrate /nitrite in each sample is then directly determined from the diazo dye absorbance at 540 nm within 45 minutes. Kit Contents, Storage and Shelf Life REAGEN™ Nitrate/Nitrite Assay Kit has the capacity for 48 determinations or 24 determinations in duplicate. Store the kit at 4°C. The shelf life of the kit is 12 months, after receipt, when the kit is properly stored. Kit Contents Amount Storage Buffer Solution 4 mL 4°C Reagent Mix 1 vial 4°C Nitrate Reductase 1 vial 4°C Color Reagent 1 3 mL 4°C Color Reagent 2 3 mL 4°C Microtiter Plate One 4°C Sensitivity (Detection Limit) Sample Volume Detection Limit (mg/L) 0.1 mL 0.02 The linear range of the assay is 0.02 –40 mg/L (ppm). Required Materials Not Provided With the Kit Spectrophotometer (578 nm) Low volume (0.3 mL) UV cuvettes, such as Brand Scientific catalog #7592 00 Deionized or distilled water 100 mL volumetric flask 50 mL plastic conical tubes Carrez buffer I and Carrez buffer II (recipes included in protocol) |
| CAS Registry Number: | RNF91004 |
| Company: | REAGEN LLC [ United States ] |
| Contact: | Nicole |
| Tel: | 8568585434 |
| Fax: | 1-302-269-1897 |
| Email: | reagenkits@gmail.com |
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