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Nitrofuran(AOZ) rapid test strip

Inquiry
  Post Date: May 04,2015
  Expiry Date: May 03,2016
  Detailed Description: Cas No. :BKU-20051 Specs:0.5ppb ,50tests/kit
Payment Method: T/T
1. Brief
This product is used for testing Nitrofuran(AOZ) residue in honey samples. The whole testing needs about 2h, suitable for all kinds of enterprises and inspection agencies.
The detection limit is 0.5ppb(ug/kg)

2. Components
1) Nitrofuran (AOZ) colloidal gold rapid test strip
(Including tablet, gold label well, dropper, desiccant) 50 pieces/kit
2) Manuals 1 piece/kit
3) 2-Nitrobenzaldehyde (C7H5NO3) 1 bottle/kit
4) Nitrofuran redissolving solution 1 bottle/kit

3. Equipments required but not provided
Micropipettes, nitrogen-drying device or air-drying device, water bath, centrifuge, balance (a reciprocal sensibility of 0.01 g);

4. Sample preparation]
1) Weigh 3g honey sample into 50ml centrifuge tube, add 4ml deionized water, 0.5ml 1M HCl, 0.2ml 2-Nitrobenzaldehyde (C7H5NO3), mix thoroughly for 3 min;
2) Incubate in water bath at 60℃ for 1h;
3) Take the tube out from the water bath, add 5ml 0.1M K2HPO4, 0.4ml 1M NaOH, 6ml ethyl acetate, mix thoroughly for 1min, centrifuge at 4000r/min for 5min;
4) Take 3ml supernatant into 5ml centrifuge tube, blow to dry by air at 60℃;
5) Add 0.5ml N-hexane and 0.3ml nitrofuran redissolving solution to the dry tube, mix evenly, centrifuge at 4000r/min for 1min (or put aside until stratification);
6) Use Micropipettes to absorb 100ul down-layer solution into gold label well, ready to test.

5. Operation procedures
1) Read the Instruction, return the reagents to room temperature (20℃~30℃) before testing.
2) Take out the test strip, put it on desk flatly, as the following picture on the right. (Use it immediately after opening the package.)
3) Absorb 100μL solution into gold label well, wait for 2 min, use small dropper to blow and beat until the red material is completely dissolved, wait to react for 2min,
4) Use dropper absorb all sample from the well, vertically add it into sample collecting well (as following picture), timing after adding sample.
5) Read the result in 5-8 minutes, otherwise it is invalid.
6. Test Result Interpretation
6.1 Negative (—): T line appears (T line, near Sample Collecting Region), T line is darker or has the same color with C line, it means the AOZ concentration is lower than detection limit or no AOZ residue.
6.2 Positive (+): T line is lighter than C line or T line is invisible, it means the AOZ concentration is higher than detection limit. The more lighter of T line compared with C line, the higher content of AOZ in sample.
6.3 Invalid: C line is invisible, maybe it is the operation error or test strip is invalid. Read the Instruction, use new test strip to test again.

7. Precautions
1) Test at 20℃~30℃ environment.
2) Wear gloves when testing.
3) Do not eat any of the reagents.
4) Do not use test card out of expiry date.
5) Do not touch the white membrane surface in the middle of test card.
6) Do not repeat use the dropper, to avoid cross pollution.

8. Precision
Use this product and AOZ Liquid-phase tandem mass spectrometry method at the same time to test 150 samples including 94 negative samples and 56 positive samples, the results show that the coincidence rate is 98.0% between this product and AOZ Liquid-phase tandem mass spectrometry method.

9. Storage and expiry date
Storage: Store at 4-30 ℃ in dark, no frozen.
Expiry date: 12 months; date of production is on box.

  CAS Registry Number:

BKU-20051


  Company: Shenzhen Biokau Sci-tech Co.,ltd         
  Contact: Samara Duan
  Tel: +86-0-13724287324
  Fax:
  Email: sales@biokau.com
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