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Nitrofuran (AOZ) ELISA Test Kit
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Post Date: | Aug 02,2017 |
Expiry Date: | Aug 02,2018 |
Detailed Description: |
Specs:96 wells/kit Payment Method: T/T or Western Union Nitrofuran (AOZ) ELISA Test Kit Catalog No. LSY-10002 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of AOZ in the sample. The coupling antigens are pre-coated on the micro-well stripes. The AOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AOZ in it. This value is compared to the standard curve and the AOZ concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.02ppb Incubation Temperature: 25℃ Incubation Time: 60min~30min Detection limit Tissue, liver 0.04 ppb Honey, milk, intestine 0.04 ppb Fish, shrimp (some interference) 0.1 ppb Cross-reaction rate AOZ 100% AMOZ <0.1% AHD <0.1% SEM <0.1% Recovery rate Tissue, liver 80±25% Honey, milk, intestine 75±17% 3. Components 1) Micro-well strips: 12 strips with 8 removable wells each 2) 6× standard solution (1 mL each): 0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb and 1.62ppb 3) Enzyme conjugate (7 mL) red cap 4) Antibody working solution (7 mL) blue cap 5) Substrate A solution (7 mL) white cap 6) Substrate B solution (7 mL) black cap 7) Stop solution (7 mL) yellow cap 8) 20× concentrated washing buffer (40 mL) white cap 9) 2× concentrated redissolving solution (50 mL) transparent cap 10) 2-Nitrobenzaldehyde (C7H5NO3)(10 mL) white cap |
Company: | Shenzhen Lvshiyuan Biotechnology Co.,Ltd [ China ] |
Contact: | Bella Zou |
Tel: | +86-755-28438788 |
Fax: | +86-755-28938800 |
Email: | info@lsybt.com |
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