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Nitrofuran (AOZ) ELISA Test Kit

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  Post Date: Aug 02,2017
  Expiry Date: Aug 02,2018
  Detailed Description: Specs:96 wells/kit
Payment Method: T/T or Western Union
Nitrofuran (AOZ) ELISA Test Kit
Catalog No. LSY-10002
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of AOZ in the sample. The coupling antigens are pre-coated on the micro-well stripes. The AOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AOZ in it. This value is compared to the standard curve and the AOZ concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.02ppb
Incubation Temperature: 25℃
Incubation Time: 60min~30min
Detection limit
Tissue, liver 0.04 ppb
Honey, milk, intestine 0.04 ppb
Fish, shrimp (some interference) 0.1 ppb
Cross-reaction rate
AOZ 100%
AMOZ <0.1%
AHD <0.1%
SEM <0.1%
Recovery rate
Tissue, liver 80±25%
Honey, milk, intestine 75±17%

3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb and 1.62ppb
3) Enzyme conjugate (7 mL) red cap
4) Antibody working solution (7 mL) blue cap
5) Substrate A solution (7 mL) white cap
6) Substrate B solution (7 mL) black cap
7) Stop solution (7 mL) yellow cap
8) 20× concentrated washing buffer (40 mL) white cap
9) 2× concentrated redissolving solution (50 mL) transparent cap
10) 2-Nitrobenzaldehyde (C7H5NO3)(10 mL) white cap


  Company: Shenzhen Lvshiyuan Biotechnology Co.,Ltd     [ China ]        
  Contact: Bella Zou
  Tel: +86-755-28438788
  Fax: +86-755-28938800
  Email: info@lsybt.com
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