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Sudan Red ELISA Test Kit
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| Post Date: | Feb 19,2023 |
| Expiry Date: | Feb 19,2024 |
| Detailed Description: |
1. Principle
Sudan red ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Sudan red in egg, feed and food. The coupling antigens are coated on the micro-well stripes. The Sudan red in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Sudan red antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Sudan red in it. The measurement is made photometrically at 450nm. The absorption is inversely proportional to the Sudan red concentration in the sample. 2. Technical specifications Sensitivity : 0.1 ppb Detect time: Approx. 75 min Detection limit: Eggs .................................................................. 10 ppb Tomato Juice / Chili sauce / Ketchup ................20 ppb Feed / Paprika..................................................100 ppb Recovery rate: Eggs ..............................................................70 ± 10% Tomato Juice/Chili sauce/Ketchup................80 ± 10% Feed / Paprika...............................................65 ± 10% Cross-reaction rate: Sudan red....................................................... 100% Para Red......................................................... 123% Rhodamine B ....................................................8% 3. Components 1) Microplate: 12 strips with 8 removable wells each 2) 6× standard solution (1 mL each): High standard（1ppm) 0ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1ppb 3) Enzyme conjugate (12 mL)...............................red cap 4) Antibody solution (7 mL)..................................green cap 5) Substrate A solution (7 mL)...............................brown cap 6) Substrate B solution (7 mL)................................black cap 7) Stop solution (7 mL)...........................................yellow cap 8) Washing solution (40 mL, 20× )..........................transparent cap 9) Redissolving solution (50 mL,2×).......................blue cap |
| Company: | Wuhan Ecalbio Co.,Ltd [ China ] |
| Contact: | Ku |
| Tel: | 027-87745856 |
| Fax: | - |
| Email: | info@ecalbio.com |
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