Trichothecenes (T-2) ELISA Test Kit
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| Post Date: | May 04,2015 |
| Expiry Date: | May 03,2016 |
| Detailed Description: |
Cas No. :BKU-10035
Quantity: 1 Specs:Sensitivity: 0.2 ppb，96wells/kit Payment Method: T/T 1. General T-2 toxin is a mycotoxin produced by various Fusarium. It mainly pollutes wheat, barley, corn and other food crops and their products, which constitute a great hazard to human health and animal husbandry. T2 mainly affects the function of the blood, liver, kidney, pancreatic muscle and lymphocyte. The clinical symptoms of T2 toxin poisoning is anorexia, vomiting, diarrhea, growth retardation, reproductive and neurological dysfunction etc.The use of Trichothecenes(T2) ELISA test kit to analyze T2 residue of samples is fast and accurate. This kit is a new generation of fungal toxins detection products in application of ELISA technology and the operation time is shorter than 60min, to minimize operating errors and work intensity. 2. Principle This test kit is based on the competitive enzyme immunoassay for the detection of T2 in the sample. The coupling antigens are pre-coated on the micro-well stripes. The T2 in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-T2 antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the T2 in it. This value is compared to the standard curve and the T2 concentration is subsequently obtained. 3. Application This test kit can be used to detect T2 in corn, rice, wheat, beans, peanuts, oats, feed and other samples qualitatively and quantitatively. 4. Technical specifications Sensitivity: 0.2 ppb Detection limit: Peanuts, corn, oats, beans, feed etc. …………………………………………………24ppb Wheat……………………………………………………………………………………..20ppb Recovery rate: 110±15% Coefficient of variation: <10% 5. Components 1) Micro-well strips: 12 strips with 8 removable wells each 2) 6 x Concentrated standards………………………………………………………1ml each 0 ppb (zero standard), 2 ppb, 6 ppb, 18 ppb, 54 ppb, 162 ppb Standard working concentration: 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb 3) T2 Enzyme conjugate ……………………………………………………………… 6ml 4) T2 Antibody working solution……………………………………………………… 6 ml 5) Substrate A....................................................................................................... 6ml 6) Substrate B....................................................................................................... 6ml 7) Stop solution......................................................................................................6ml 8) Concentrated washing buffer（10×）......................................................... ..40ml 6. Materials required but not provided 1) Equipment: microtiter plate spectrophotometer (450 nm/630nm), oscillator, vortex shaker, centrifuge, balance: 0.01g quantity sensitive, gaduated pipettes: 25ml, ear wash ball, funnel, triangle bottle with plug, polystyrene centrifuge tube: 10ml,50ml, quantitative analysis of filter paper 2) Micropipettes: single-channel 10~100µl, 200~1000µl, multi-channel 250µl 3) Reagents: methanol（analysis pure）, deionized water 7. Sample pre-treatment Solution preparation before sample pre-treatment: 1) washing buffer Use 1 part of concentrated washing buffer（10×） and dissolve with 9 parts of deionized water to obtain the ready to use washing buffer. Solution is stable for 1 month when stored at 4 °C. 2) sample extract solution Use 3 part of methanol and dissolve with 2 parts of deionized water to obtain the ready to use sample extract. 3) 6 T2 standards Take six 1.5ml centrifuge tubes, numbered from 1 to 6. Take six concentrations of standard (10 ×) (0ppb, 2ppb, 6ppb, 18ppb, 54ppb, 162ppb) 50μl ,add to the above six tubes, then each tube adding 450μl deionized water to dilute them by 1: 9, obtain 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb the standard solution(see the following table). Configured standard solution should be used in one week (4 ℃ storage) Tube No. Standard working concentration (ppb) Standard (10X) concentration (ppb) Standard (10X) volume Deionized water volume 1 0 1 50µl + 450µl 2 0.2 2 50µl + 450µl 3 0.6 6 50µl + 450µl 4 1.8 18 50µl + 450µl 5 5.4 54 50µl + 450µl 6 16.2 162 50µl + 450µl Samples Preparation 7.1 Preparation of Peanuts, corn, oats, beans, feed etc. − weigh 1g of ground sample into a suitable container and add 20 ml sample extract solution − shake vigorously for five minutes (manually or with shaker) − filter the extract through filter − dilute 1 part of the obtained filtrate with 5 part of distilled water or deionized water. − take the diluted liquid to test Dilution factor: 120 7.2 Preparation of wheat sample − weigh 1g of ground sample into a suitable container and add 20ml of sample extract solution − shake vigorously for 5 minutes (manually or with shaker) − filter the extract through filter − take 1ml supernatant, add 2ml methylene dichloride, shake, centrifuge for 5min at 4000r/min, take methylene dichloride layer, blow to dryness at 50℃. − add 5 ml 10% methanol, shake evenly. − take the liquid to test Dilution factor: 100 |
| CAS Registry Number: | BKU-10035 |
| Company: | Shenzhen Biokau Sci-tech Co.,ltd |
| Contact: | Samara Duan |
| Tel: | +86-0-13724287324 |
| Fax: | |
| Email: | sales@biokau.com |
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