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Ammonia Assay Kit

  • Post Date:

    Sep 10,2015
  • Expiry Date:

    Sep 09,2016
  • Detailed Description:

    Cas No. :RNF91003 Product Description

    REAGEN™ Ammonia Assay Kit is a cuvette-based enzymatic assay for the determination of Ammonia across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Ammonia directly from food samples.
    The unique features of the kit are:

     High sensitivity and low detection limit (0.2 ppm)
     High reproducibility
     A rapid (30 minute), robust enzyme-based assay

    Procedure Overview

    REAGEN™ Ammonia Assay Kit uses a coupled enzymatic reaction scheme to detect Ammonia in food samples. The ammonia is used as a substrate by the enzyme glutamate dehydrogenase to make glutamate. In the process, NADH is stoichiometrically converted to NAD+. This conversion, measured at 340 nm, is proportional to the level of Ammonia in the sample.

    The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of Ammonia in each sample is then directly determined from the change in NADH absorbance at 340 nm within 25 minutes.

  • CAS Registry Number:

    RNF91003
  • Company:

    REAGEN LLC     [ United States ]        
  • Contact:

    nicole
  • Tel:

    8566711789
  • Fax:

    8568585434
  • Email:

    reagenllc@gmail.com
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