Avian Marek's disease virus antibody ELISA kit

• Post Date:

  Aug 02,2017

• Expiry Date:

  Aug 02,2018

• Detailed Description:

  Specs:96 wells/kit
  Price:200 USD Metric Tons
  Payment Method: T/T or Western Union
  Avian Marek's disease virus antibody ELISA kit
  Catalog No. LSY-30016
  
  1. Usage
  This kit is used to detect Avian Marek's disease virus antibody in chicken serum, to assess antibody condition by Avian Marek's disease vaccine in chicken farm and assist diagnosis of serological infected chicken.
  
  2. Principle
  The Avian Marek's disease antibody ELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (450 nm).
  
  3. Reagents
  1) MD antigen coated microtiter strips 1 piece (96 wells)
  2) Conjugate solution 1 bottle (12 mL/bottle)
  3) 10× washing solution………………………………………1 bottle (50 mL/bottle)
  4) Substrate A /B solution 1 tube each (6 mL/tube)
  5) Sample dilution……………………………………………. 1 bottle (50 mL/bottle)
  6) Stopping solution 1 tube (6 mL/tube)
  7) Negative control 1 tube (800μL /tube)
  8) Positive control 1 tube (800μL /tube)
  9) Serum dilution plate……………………………………………………..…..1 piece
  10) Adhesive film……………………………………………………………….2 pieces
  11) Instruction……………………………………………………………………1 piece
  
  4. Materials required but not provided
  1) Micropipettors and disposable tips: 0.5μL~10μL、10μL~100μL、100μL~1000μL
  2) 37 ℃ Incubator
  3) Measuring cylinder: 500 ml
  4) 96 wells microplate reader
  5) Distilled water or deionied water
  6) Bottle or microplate washing machine
  
  5. Sample preparation
  Take animal whole blood, make serum according to regular methods, the serum should be clear, have no hemolysis.
  

• Company:

  Shenzhen Lvshiyuan Biotechnology Co.,Ltd     [ China ]        

• Contact:

  Bella Zou

• Tel:

  +86-755-28438788

• Fax:

  +86-755-28938800

• Email:

  info@lsybt.com