Bisphenol A (BPA) ELISA Test Kit

• Post Date:

  Feb 19,2023

• Expiry Date:

  Feb 19,2024

• Detailed Description:

  Principle
  Bisphenol A (BPA) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Bisphenol A in tissue,honey, milk, urine and Serum.
  The coupling antigens are coated on the micro-well stripes. The Bisphenol A in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Bisphenol A antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
  The optical density (OD) value of the sample has a negative correlation with the Bisphenol A in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Bisphenol A concentration in the sample.
  
  Technical specification:
  Sensitivity: 5 ppb Detect time: Approx.75 min
  
  Detect limit
  Urine,serum.................................... 10 ppb
  Liver,tissue......................................7.5 ppb
  
  Recovery rate
  Urine,serum................................ 70 ± 10%
  Liver,tissue..................................80 ± 10%
  
  Cross-reaction rate
  Bisphenol A .......................100%
  Thiamphenicol........................< 0.1%
  Florfenicol...............................< 0.1%
  
  Components
  1.Microplate: 12 strips with 8 removable wells each
  2.6× standard solution (1 mL each):
  0 ppb, 1ppb, 5 ppb, 15 ppb, 45 ppb, 135 ppb, 405 ppb,
  3.Enzyme conjugate (1................................................... red cap
  4.Antibody working solution (7 mL)......................................green cap
  5.Substrate A solution (7 mL)......................................... white cap
  6.Substrate B solution (7 mL).......................................... black cap
  7.Stop solution (7 mL)................................................ yellow cap
  8.Washing solution (40 mL, 20× ) ............................. transparent cap
  9.Redissolving solution (50 mL, 2× )................................... blue cap

• Company:

  Wuhan Ecalbio Co.,Ltd     [ China ]        

• Contact:

  Ku

• Tel:

  027-87745856

• Fax:

  -

• Email:

  info@ecalbio.com