Cephalosporins ELISA Kit

• Post Date:

  Feb 19,2023

• Expiry Date:

  Feb 19,2024

• Detailed Description:

  1. Principle
  This test kit is based on the competitive enzyme immunoassay for the detection of Cephalosporins in the tissue, liver, honey, intestine, shrimp, fish, milk, etc. The coupling antigens are pre-coated on the micro-well stripes. The Cephalosporins in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Cephalosporins antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Cephalosporins concentration in the sample. This value is compared to the standard curve and the content of the corresponding Cephalosporins is subsequently obtained.
  
  2. Technical specifications
  Sensitivity : 1 ppb Detect time: Approx. 75 min
  
  Detection limit:
  Tissue..............................4 ppb
  Milk ..............................40 ppb
  
  Recovery rate:
  Tissue, milk.................85 ±10%
  
  Cross-reaction rate:
  Cefsulodin ....................100%
  Cephalexin  ................... 70%
  Cefuroxim  ....................35%
  
  3. Components
  1）Microplate: 12 strips with 8 removable wells each
  2）6× standard solution (1 mL each):0 ppb, 1ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb
  3）Enzyme conjugate (7 mL)..................................red cap
  4）Antibody solution (7 mL).................................green cap
  5）Substrate A solution (7 mL)..............................brown cap
  6）Substrate B solution (7 mL)..............................black cap
  7）Stop solution (7 mL).....................................yellow cap
  8）Washing solution (40 mL 20× )...........................white cap
  9）Redissolving solution (50 mL 2×)........................blue cap

• Company:

  Wuhan Ecalbio Co.,Ltd     [ China ]        

• Contact:

  Ku

• Tel:

  027-87745856

• Fax:

  -

• Email:

  info@ecalbio.com