Erythromycin ELISA Test Kit

• Post Date:

  Feb 19,2023

• Expiry Date:

  Feb 19,2024

• Detailed Description:

  1. Principle
  Erythromycin ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Erythromycin in milk, honey and meat.The coupling antigens are coated on the micro-well stripes. The Erythromycin in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Erythromycin antibodies.After the addition of the enzyme conjugate, the TMB substrate is added for coloration.The optical density (OD) value of the sample has a negative correlation with the Erythromycin in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Erythromycin concentration in the sample.
  
  2. Technical specification:
  Sensitivity: 0.1 ppb Detect time: Approx.75 min
  
  Detect limit:
  Chicken,duck, pork, shrimp,fish,honey and milk .................4 ppb
  
  Cross-reaction rate:
  Erythromycin.............................................................. 100%
  Erythromycin Thiocyanate ................................................ 105%
  Erythromycin Ethylsuccinate ............................................ 85%
  
  Recovery rate:
  Tissue ............................................................... 85 ± 20%
  Honey, milk .......................................................... 75 ± 20%
  
  3. Components
  1） Microplate: 12 strips with 8 removable wells each
  2） 6× standard solution (1 mL each): 0ppb、0.1 ppb、0.3 pb、 0.9 ppb、2.7 ppb、8.1ppb
  3） Enzyme conjugate (12 mL) ............................................ red cap
  4） Antibody solution (7 mL) ........................................ green cap
  5） Substrate A solution (7 mL) ..................................... white cap
  6） Substrate B solution (7 mL) ....................................... black cap
  7） Stop solution (7 mL)............................................. yellow cap
  8） Washing solution (40 mL, 20× Concentrate).................transparent cap
  9） Buffer 1 (50 mL, 2× Concentrate)..................................blue cap

• Company:

  Wuhan Ecalbio Co.,Ltd     [ China ]        

• Contact:

  Ku

• Tel:

  027-87745856

• Fax:

  -

• Email:

  info@ecalbio.com