Fructose/Glucose Assay Kit

• Post Date:

  Sep 10,2015

• Expiry Date:

  Sep 09,2016

• Detailed Description:

  Cas No. :RNF91002 Product Description
  
  REAGEN™ Fructose/Glucose Assay Kit is a cuvette-based enzymatic assay for the determination of Fructose/Glucose across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Fructose/Glucose directly from food samples.
  The unique features of the kit are:
  
   High sensitivity and low detection limit (0.8 ppm)
   High reproducibility
   A rapid (30 minute), robust enzyme-based assay
  
  Procedure Overview
  
  REAGEN™ Fructose/Glucose Assay Kit uses a coupled enzymatic reaction to detect glucose and/or fructose in food samples: glucose is detected by phosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). This compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of glucose in the sample. Fructose is detected in similar manner after it is specifically converted to glucose-6-phosphate (G-6-P) using the enzyme phosphoglucose isomerase (PGI).
  
  The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of glucose in each sample is then directly determined from the change in absorbance at 340 nm within 30 minutes.
  
  

• CAS Registry Number:

  RNF91002

• Company:

  REAGEN LLC     [ United States ]        

• Contact:

  nicole

• Tel:

  8566711789

• Fax:

  8568585434

• Email:

  reagenllc@gmail.com