Fructose/Glucose Assay Kit

• Post Date:

  Sep 10,2015

• Expiry Date:

  Sep 09,2016

• Detailed Description:

  Cas No. :RNF91002 Specs:95%
  Payment Method: tt
  Product Description
  
  REAGEN™ Fructose/Glucose Assay Kit is a cuvette-based enzymatic assay for the determination of Fructose/Glucose across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Fructose/Glucose directly from food samples.
  The unique features of the kit are:
  
   High sensitivity and low detection limit (0.8 ppm)
   High reproducibility
   A rapid (30 minute), robust enzyme-based assay
  
  Procedure Overview
  
  REAGEN™ Fructose/Glucose Assay Kit uses a coupled enzymatic reaction to detect glucose and/or fructose in food samples: glucose is detected by phosphorylation with hexokinase to make glucose-6-phosphate (G-6-P). This compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of glucose in the sample. Fructose is detected in similar manner after it is specifically converted to glucose-6-phosphate (G-6-P) using the enzyme phosphoglucose isomerase (PGI).
  
  The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of glucose in each sample is then directly determined from the change in absorbance at 340 nm within 30 minutes.
  
  Kit Contents, Storage and Shelf Life
  
  REAGEN™ Fructose/Glucose Assay Kit has the capacity for 48 determinations or 24 determinations in duplicate. Store the kit at 4°C. The shelf life of the kit is 12 months, after receipt, when the kit is properly stored.
  
  
  Kit Contents Amount Storage
  Dilution Buffer 10 mL 4ºC
  Substrate Mix 20 mL 4ºC
  Hexokinase 0.7 mL 4ºC
  PGI Enzyme 0.2 mL 4ºC
  Microtiter Plate One 4ºC
  
  Sensitivity (Detection Limit)
  
  Sample Volume Detection Limit (mg/L)
  0.01 mL 8
  0.1mL 0.8
  
  The linear range of the assay is 0.8 – 1000 mg/L (ppm).
  
  Required Materials Not Provided With the Kit
  
   Spectrophotometer (340 nm)
   Low volume (0.3 mL) UV cuvettes, such as Brand Scientific catalog #7592 00
   Deionized or distilled water
   50 mL plastic conical tubes
  
  Warnings and Precautions
  
   Do not use the kit past the expiration date.
   Try to maintain a laboratory temperature of 20°–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays in direct sunlight, as this may cause excessive heat and evaporation. Cold bench tops should be avoided by placing several layers of paper towel or some other insulate material under the assay plates during incubation.
   Make sure you are using only distilled or deionized water since water quality is very important.
   When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic.
  
  
  

• CAS Registry Number:

  RNF91002

• Company:

  REAGEN LLC     [ United States ]        

• Contact:

  Nicole

• Tel:

  8568585434

• Fax:

  1-302-269-1897

• Email:

  reagenkits@gmail.com