HPLC Columns

• Post Date:

  Jul 30,2013

• Expiry Date:

  Jul 30,2014

• Detailed Description:

  Payment Method: HPLC Columns
  Detailed Product Description
  
  
  Fuji padding has stable performance, lower column pressure,column efficiency moderate, high cost performance .
  
  
  HPLC columns are packed with very fine particles (usually a few microns in diameter). The very fine particles are required to attain the low dispersion that give the high plate counts expected of modern HPLC.
  
  
  
  Plate counts in excess of 25,000 plates per column are possible with modern columns, however, these very high efficiencies are very rarely found with real samples because of the dispersion associated with injection valves, detectors, data acquisition systems and the dispersion due to the higher molecular weight of real samples as opposed to the common test samples.
  
  
  
  Packing these small particles into the column is a difficult technical problem but even with good packing a great amount of care must be given to the column end fittings and the inlet and outlet connection to keep dispersion to a minimum. Some state of the art systems are now 'chip' based and may use no particles at all. Some limited use has been made of HPLC for preparative purposes using half inch to one inch diameter columns.
  
  
  
  LC columns, in general, achieve their separation by exploiting the different intermolecular forces between the solute and the stationary pahse and those between the solute and the mobile phase. The column will retain those substances that interact more strongly with the stationary pahse than those that interact more strongly with the mobile phase. The basic intermolecular forces that are exploited in the HPLC are the same as those discussed in The Mechanism of Chromatographic Retention and The Thermodynamics of Chromatography of the Chrom-Ed series.
  
  
  
  The main consideration with HPLC is the much wider variety of solvents and packing materials that can be utilized as because of the much lower quantities of both which are required. In particular very expensive optically pure compounds can be used to make Chiral HPLC stationary phases and may even be used as (disposable) HPLC solvents.
  
  

• Company:

  Dalian Replete Science And Technology CO.,LTD     [ China ]        

• Contact:

  Helen Sun

• Tel:

  0411-83786041

• Fax:

  0411-83786043

• Email:

  83789041@163.com