Human VB12 ELISA Kit

Post Date:
Jul 14,2020
Expiry Date:
Jan 10,2021
Detailed Description:
Purpose
This kit allows for the determination of VB12 concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human VB12 level in the sample，use Purified Human VB12 antibody to coat microtiter plate wells, make solid-phase antibody, then add VB12 to wells, Combined VB12 antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VB12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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