Malachite green ELISA kit
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Post Date:
Aug 02,2017
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Expiry Date:
Aug 02,2018
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Detailed Description:
Payment Method: T/T or Western Union
Malachite green ELISA kit
Catalog No. LSY-10027
1. Principle
This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Malachite green in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Malachite green antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Malachite green in it. This value is compared to the standard curve and the content of Malachite green residues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.05 ppb
Incubator temperature: 25℃
Incubator time: 30min~30min~15min
Detection limit
Aquatic products e.g. Shrimp, Fish 0.5 ppb
Water 0.1 ppb
Recovery rate
Shrimp, Fish, water 80%--110%
Cross-reaction rate
Malachite green 100%
Leucomalachite green 0.1%
Gentian violet 95%
Leucocrystal violet 0.1%
3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each, white cap): 0 ppb, 0.05 ppb, 0.15 ppb,
0.45 ppb, 1.35 ppb and 4.05 ppb
3) Enzyme conjugate (12 mL) red cap
4) Antibody working solution (7 mL) blue cap
5) Substrate A solution (7 mL) white cap
6) Substrate B solution (7 mL)……………………………………………. black cap
7) Stop solution (7 mL) yellow cap
8) 20× concentrated washing buffer (40 mL) white cap
9) Oxidant (3 mL) black cap
10) 2× concentrated redissolving solution (50 mL) transparent cap
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Company:
Shenzhen Lvshiyuan Biotechnology Co.,Ltd
[ China ]
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Contact:
Bella Zou
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Tel:
+86-755-28438788
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Fax:
+86-755-28938800
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Email:
info@lsybt.com
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