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Malachite green ELISA kit

  • Post Date:

    Aug 02,2017
  • Expiry Date:

    Aug 02,2018
  • Detailed Description:

    Payment Method: T/T or Western Union
    Malachite green ELISA kit
    Catalog No. LSY-10027
    1. Principle
    This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Malachite green in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Malachite green antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Malachite green in it. This value is compared to the standard curve and the content of Malachite green residues is subsequently obtained.

    2. Technical specifications
    Sensitivity: 0.05 ppb
    Incubator temperature: 25℃
    Incubator time: 30min~30min~15min
    Detection limit
    Aquatic products e.g. Shrimp, Fish 0.5 ppb
    Water 0.1 ppb
    Recovery rate
    Shrimp, Fish, water 80%--110%
    Cross-reaction rate
    Malachite green 100%
    Leucomalachite green 0.1%
    Gentian violet 95%
    Leucocrystal violet 0.1%

    3. Components
    1) Micro-well strips: 12 strips with 8 removable wells each
    2) 6× standard solution (1 mL each, white cap): 0 ppb, 0.05 ppb, 0.15 ppb,
    0.45 ppb, 1.35 ppb and 4.05 ppb
    3) Enzyme conjugate (12 mL) red cap
    4) Antibody working solution (7 mL) blue cap
    5) Substrate A solution (7 mL) white cap
    6) Substrate B solution (7 mL)……………………………………………. black cap
    7) Stop solution (7 mL) yellow cap
    8) 20× concentrated washing buffer (40 mL) white cap
    9) Oxidant (3 mL) black cap
    10) 2× concentrated redissolving solution (50 mL) transparent cap
  • Company:

    Shenzhen Lvshiyuan Biotechnology Co.,Ltd     [ China ]        
  • Contact:

    Bella Zou
  • Tel:

    +86-755-28438788
  • Fax:

    +86-755-28938800
  • Email:

    info@lsybt.com
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