Melamine ELISA Test Kit

• Post Date:

  Aug 02,2017

• Expiry Date:

  Aug 02,2018

• Detailed Description:

  Payment Method: T/T or Western Union
  Melamine ELISA Test Kit
  Catalog No. LSY-10026
  1. Principle
  This test kit is based on the competitive enzyme immunoassay for the detection of Melamine in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Melamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Melamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Melamine concentration in the sample. This value is compared to the standard curve and the Melamine concentration is subsequently obtained.
  
  2. Technical specifications
  Sensitivity: 1 ppb
  Incubator temperature: 25℃
  Incubator time: 30min～15min
  Detection limit:
  Milk powder 20 ppb
  Milk 16 ppb
  Milk/Milk powder (Method two) 1 ppb
  Tissue (chicken, pork, duck, fish, shrimp and liver) 2 ppb
  Feed 100 ppb
  Egg 20 ppb
  Serum 4 ppb
  Cross-reaction rate:
  Melamine 100%
  Cyanuric acid 60%
  Triazine <1%
  Diamino atrazine <1%
  Recovery rate:
  Milk/Milk powder 90±19%
  Tissue 85±20%
  Feed 85±20%
  Egg 80±20%
  
  3. Components
  1） Micro-well strips: 12 strips with 8 removable wells each
  2） 6× standard solution (1 mL each): 0 ppb,1 ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb
  3） Enzyme conjugate (7 mL) red cap
  4） Antibody working solution (7 mL) blue cap
  5） Substrate A solution (7 mL) white cap
  6） Substrate B solution (7 mL) black cap
  7） Stop solution (7 mL) yellow cap
  8） 20× concentrated washing buffer (40 mL) white cap
  9） 2× concentrated redissolving solution (50 mL) transparent cap
  

• Company:

  Shenzhen Lvshiyuan Biotechnology Co.,Ltd     [ China ]        

• Contact:

  Bella Zou

• Tel:

  +86-755-28438788

• Fax:

  +86-755-28938800

• Email:

  info@lsybt.com