Neomycin (NM) ELISA kit

• Post Date:

  Feb 19,2023

• Expiry Date:

  Feb 19,2024

• Detailed Description:

  1. Principle
  This test kit is based on the competitive enzyme immunoassay for the detection of Neomycin in the tissue, liver, honey, intestine, shrimp, fish, etc.The coupling antigens are pre-coated on the micro-well stripes. The Neomycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Neomycin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Neomycin concentration in the sample. This value is compared to the standard curve and the content of the corresponding Neomycin is subsequently obtained.
  
  2. Technical specifications
  Sensitivity : 0.5 ppb Detect time: Approx. 75 min
  
  Detection limit:
  Tissue, liver, honey, shrimp, fish, milk ........... 10 ppb
  
  Recovery rate:
  Tissue, liver, honey, shrimp, fish, milk....... 85 ±10%
  
  Cross-reaction rate:
  Neomycin .......................100%
  Streptomycin................... < 1 %
  Gentamicin ..................... < 1 %
  
  3. Components
  1）Microplate: 12 strips with 8 removable wells each
  2）6× standard solution (1 mL each):
  0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb, 40.5 ppb
  3）Enzyme conjugate (12 mL)................................red cap
  4）Antibody solution (7 mL)................................ green cap
  5）Substrate A solution (7 mL)............................. brown cap
  6）Substrate B solution (7 mL)............................. black cap
  7）Stop solution (7 mL)........................................ yellow cap
  8）Washing solution (40 mL 20× )........................ white cap
  9）Redissolving solution (50 mL 2×)..................... blue cap

• Company:

  Wuhan Ecalbio Co.,Ltd     [ China ]        

• Contact:

  Ku

• Tel:

  027-87745856

• Fax:

  -

• Email:

  info@ecalbio.com