Nitiomidazoles ELISA Kit

• Post Date:

  Feb 19,2023

• Expiry Date:

  Feb 19,2024

• Detailed Description:

  1. Principle
  This test kit is based on the competitive enzyme immunoassay for the detection of nitiomidazoles in the tissue, liver, honey, intestine, shrimp, fish, etc. The coupling antigens are pre-coated on the micro-well stripes. The nitiomidazoles in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Gen antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density(OD) value of the sample has a negative correlation with nitiomidazoles concentration in the sample. This value is compared to the standard curve and the content of the corresponding nitiomidazoles is subsequently obtained.
  
  2. Technical specifications
  Sensitivity : 1 ppb Detect time: Approx. 75 min
  
  Detection limit:
  Tissue, liver, intestine,shrimp,fish,honey .......... 3 ppb
  Milk ................................................................... 40 ppb
  
  Recovery rate:
  Tissue, liver, honey, intestine,shrimp,fish,milk ....... 80 ±10%
  Milk ................................................................ 85 ±10%
  
  Cross-reaction rate:
  Metronidazole.................. 100 %
  Tinidazole ....................... 90 %
  Ornidazole ..................... 80 %
  Secnidazole...................... 65 %
  
  3. Components
  1）Microplate: 12 strips with 8 removable wells each
  2）6× standard solution (1 mL each): 0 ppb, 1ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb
  3）Enzyme conjugate (7mL).....................................red cap
  4）Antibody solution (7 mL).....................................blue cap
  5）Substrate A solution (7 mL)................................. brown cap
  6）Substrate B solution (7 mL)..................................black cap
  7）Stop solution (7 mL) ......................................... yellow cap
  8）Washing solution (40 mL 20× )............................white cap
  9）Redissolving solution (50 mL 2×).........................blue cap

• Company:

  Wuhan Ecalbio Co.,Ltd     [ China ]        

• Contact:

  Ku

• Tel:

  027-87745856

• Fax:

  -

• Email:

  info@ecalbio.com