Nitrate/Nitrite Assay Kit
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Post Date:
Sep 10,2015
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Expiry Date:
Sep 09,2016
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Detailed Description:
Cas No. :RNF91004
Product Description
REAGEN™ Nitrate/Nitrite Assay Kit is a cuvette-based enzymatic assay for the determination of Nitrate/Nitrite across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Nitrate/Nitrite directly from food samples.
The unique features of the kit are:
High sensitivity and low detection limit (0.02 ppm)
High reproducibility
A rapid (45 minute), robust enzyme-based assay
Procedure Overview
REAGEN™ Nitrate/Nitrite Assay Kit uses a coupled enzymatic reaction scheme to detect nitrate and/or nitrite in food samples: nitrate in the sample is converted by nitrate reductase enzyme to make nitrite. The nitrite then reacts with sulfanilamide and N-(1-naphthyl)-ethylene-diamine to form a red diazo dye. This dye, measured at 540 nm, is proportional to the level of nitrate and/or nitrite in the sample.
The absorbance of each sample at 540 nm is measured using a spectrophotometer. The concentration of nitrate /nitrite in each sample is then directly determined from the diazo dye absorbance at 540 nm within 45 minutes.
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CAS Registry Number:
RNF91004
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Company:
REAGEN LLC
[ United States ]
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Contact:
nicole
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Tel:
8566711789
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Fax:
8568585434
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Email:
reagenllc@gmail.com
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