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Nitrate/Nitrite Assay Kit

  • Post Date:

    Sep 10,2015
  • Expiry Date:

    Sep 09,2016
  • Detailed Description:

    Cas No. :RNF91004 Product Description
    REAGEN™ Nitrate/Nitrite Assay Kit is a cuvette-based enzymatic assay for the determination of Nitrate/Nitrite across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Nitrate/Nitrite directly from food samples.
    The unique features of the kit are:
     High sensitivity and low detection limit (0.02 ppm)
     High reproducibility
     A rapid (45 minute), robust enzyme-based assay

    Procedure Overview
    REAGEN™ Nitrate/Nitrite Assay Kit uses a coupled enzymatic reaction scheme to detect nitrate and/or nitrite in food samples: nitrate in the sample is converted by nitrate reductase enzyme to make nitrite. The nitrite then reacts with sulfanilamide and N-(1-naphthyl)-ethylene-diamine to form a red diazo dye. This dye, measured at 540 nm, is proportional to the level of nitrate and/or nitrite in the sample.

    The absorbance of each sample at 540 nm is measured using a spectrophotometer. The concentration of nitrate /nitrite in each sample is then directly determined from the diazo dye absorbance at 540 nm within 45 minutes.
  • CAS Registry Number:

    RNF91004
  • Company:

    REAGEN LLC     [ United States ]        
  • Contact:

    nicole
  • Tel:

    8566711789
  • Fax:

    8568585434
  • Email:

    reagenllc@gmail.com
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