Nitrofuran (AOZ) ELISA Test Kit

• Post Date:

  Aug 02,2017

• Expiry Date:

  Aug 02,2018

• Detailed Description:

  Specs:96 wells/kit
  Payment Method: T/T or Western Union
  Nitrofuran (AOZ) ELISA Test Kit
  Catalog No. LSY-10002
  1. Principle
  This test kit is based on the competitive enzyme immunoassay for the detection of AOZ in the sample. The coupling antigens are pre-coated on the micro-well stripes. The AOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AOZ in it. This value is compared to the standard curve and the AOZ concentration is subsequently obtained.
  
  2. Technical specifications
  Sensitivity: 0.02ppb
  Incubation Temperature: 25℃
  Incubation Time: 60min～30min
  Detection limit
  Tissue, liver 0.04 ppb
  Honey, milk, intestine 0.04 ppb
  Fish, shrimp (some interference) 0.1 ppb
  Cross-reaction rate
  AOZ 100%
  AMOZ <0.1%
  AHD <0.1%
  SEM <0.1%
  Recovery rate
  Tissue, liver 80±25%
  Honey, milk, intestine 75±17%
  
  3. Components
  1） Micro-well strips: 12 strips with 8 removable wells each
  2） 6× standard solution (1 mL each): 0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb and 1.62ppb
  3） Enzyme conjugate (7 mL) red cap
  4） Antibody working solution (7 mL) blue cap
  5） Substrate A solution (7 mL) white cap
  6） Substrate B solution (7 mL) black cap
  7） Stop solution (7 mL) yellow cap
  8） 20× concentrated washing buffer (40 mL) white cap
  9） 2× concentrated redissolving solution (50 mL) transparent cap
  10） 2-Nitrobenzaldehyde (C7H5NO3)(10 mL) white cap
  

• Company:

  Shenzhen Lvshiyuan Biotechnology Co.,Ltd     [ China ]        

• Contact:

  Bella Zou

• Tel:

  +86-755-28438788

• Fax:

  +86-755-28938800

• Email:

  info@lsybt.com