Quinolones(QNS) ELISA Kit

• Post Date:

  Aug 02,2017

• Expiry Date:

  Aug 02,2018

• Detailed Description:

  Payment Method: T/T or Western Union
  Quinolones(QNS) ELISA Kit
  Catalog No. LSY-10016
  1. Principle
  This test kit is based on the competitive enzyme immunoassay for the detection of Quinolones in samples. The coupling antigens are pre-coated on the micro-well stripes. The Quinolones in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Quinolones antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Quinolones in the sample. This value is compared to the standard curve and the Quinolones concentration is subsequently obtained.
  2. Technical specifications
  Sensitivity: 1 ppb
  Incubation Temperature: 25℃
  Incubation Time: 30min—15min
  Detection limit
  Enrofloxacin (ENR) 1ppb
  Ciprofloxacin (CIF) about 1ppb
  Ofloxacin (OFL) about 1ppb
  Danofloxacin (DAN) about 1ppb
  Norfloxacin (NOR) about 0.5ppb
  Lomefloxacin (LOM) about 1ppb
  Pefloxacin (PEF) about 0.5ppb
  Enoxacin (ENO) about 1ppb
  Aosuo Li acid (OXO) about 1ppb
  Fluoroquinolone acid (FLU) about 1ppb
  Ma Paul Sand Star (MAR) about 1ppb
  Ammonia difloxacin (AMI) about 1ppb
  That difloxacin (NAD) about 2ppb
  Cross-reaction rate:
  Enrofloxacin (ENR) 100%
  Ciprofloxacin (CIF) 92.88%
  Ofloxacin (OFL) 98.86%
  Aosuo Li acid (OXO) 116.86%
  Danofloxacin (DAN) 102.63%
  Norfloxacin (NOR) 148.65%
  Lomefloxacin (LOM) 86.24%
  Pefloxacin (PEF) 156.60%
  Enoxacin (ENO) 98.97%
  Fluoroquinolone acid (FLU) 96.73%
  Ma Paul Sand Star (MAR) 110.63%
  Ammonia difloxacin (AMI) 98.86%
  That difloxacin (NAD) 56.81%
  Recovery rate
  Tissue 80±15%
  Serum 80±15%
  Honey 75±15%
  
  3. Components
  1） Micro-well strips: 12 strips with 8 removable wells each
  2） 6× standard solution (1 mL each): 0ppb, 1ppb, 3ppb, 9ppb, 27ppb and 81ppb
  3） Enzyme conjugate (7ml) ………………………………………………….red cap
  4） Antibody working solution (7 mL) blue cap
  5） Substrate A solution (7 mL) white cap
  6） Substrate B solution (7 mL) black cap
  7） Stop solution (7 mL) yellow cap
  8） 20× concentrated washing buffer (40 mL) white cap
  9） 2× concentrated redissolving solution (50 mL) transparent cap
  

• Company:

  Shenzhen Lvshiyuan Biotechnology Co.,Ltd     [ China ]        

• Contact:

  Bella Zou

• Tel:

  +86-755-28438788

• Fax:

  +86-755-28938800

• Email:

  info@lsybt.com