Tetrodotoxin ELISA Kit
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Post Date:
Feb 19,2023
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Expiry Date:
Feb 19,2024
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Detailed Description:
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Tetrodotoxin in the tissue, liver, fish. The coupling antigens are pre-coated on the micro-well stripes. The tetrodotoxin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-TTX antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Tetrodotoxin concentration in the sample. This value is compared to the standard curve and the content of the corresponding Tetrodotoxin is subsequently obtained.
2. Technical specifications
Sensitivity : 8 ppb Detect time: Approx. 75 min
Detection limit:
Tissue, liver......................... 8 ppb
Recovery rate:
Tissue, liver........................90 ± 10%
Cross-reaction rate:
Tetrodotoxin...................... 100%
3. Components
1)Microplate: 12 strips with 8 removable wells each
2)6× Standard Solution (1 mL each):
0 ppb, 8 ppb, 16 ppb, 32 ppb, 64 ppb, 128 ppb
3)Antibody Solution /Enzyme Conjugated Antibody (5µL 10000× )
4)Substrate Solution (12 mL)
5)Stop Solution (7 mL)
6)Washing Solution (30 mL 20× )
7)PBS Solution ( 10 mL )
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Company:
Wuhan Ecalbio Co.,Ltd
[ China ]
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Contact:
Ku
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Tel:
027-87745856
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Fax:
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Email:
info@ecalbio.com
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