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Tetrodotoxin ELISA Kit

  • Post Date:

    Feb 19,2023
  • Expiry Date:

    Feb 19,2024
  • Detailed Description:

    1. Principle
    This test kit is based on the competitive enzyme immunoassay for the detection of Tetrodotoxin in the tissue, liver, fish. The coupling antigens are pre-coated on the micro-well stripes. The tetrodotoxin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-TTX antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Tetrodotoxin concentration in the sample. This value is compared to the standard curve and the content of the corresponding Tetrodotoxin is subsequently obtained.

    2. Technical specifications
    Sensitivity : 8 ppb Detect time: Approx. 75 min

    Detection limit:
    Tissue, liver......................... 8 ppb

    Recovery rate:
    Tissue, liver........................90 ± 10%

    Cross-reaction rate:
    Tetrodotoxin...................... 100%

    3. Components
    1)Microplate: 12 strips with 8 removable wells each
    2)6× Standard Solution (1 mL each):
    0 ppb, 8 ppb, 16 ppb, 32 ppb, 64 ppb, 128 ppb
    3)Antibody Solution /Enzyme Conjugated Antibody (5µL 10000× )
    4)Substrate Solution (12 mL)
    5)Stop Solution (7 mL)
    6)Washing Solution (30 mL 20× )
    7)PBS Solution ( 10 mL )
  • Company:

    Wuhan Ecalbio Co.,Ltd     [ China ]        
  • Contact:

    Ku
  • Tel:

    027-87745856
  • Fax:

    -
  • Email:

    info@ecalbio.com
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